Was supported by Grants from National Simple Analysis System of China (973 Plan: 2011CB503806 to Long-xian Cheng) and National All-natural Science Foundation of China (nos. 81172750 and 81370324 to Long-xian Cheng).
Zhou et al. BMC Cancer 2013, 13:359 http://biomedcentral/1471-2407/13/RESEARCH ARTICLEOpen AccessClinical and experimental research concerning the expression and diagnostic value of carcinoembryonic antigen-related cell adhesion molecule 1 in non-small-cell lung cancerMu-qing Zhou1, Yan Du1, Yi-wen Liu2, Ying-zhi Wang1, Yi-qing He2, Cui-xia Yang2, Wen-juan Wang1 and Feng Gao1,2*AbstractBackground: Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is often a multifunctional Ig-like cell adhesion molecule that has a wide selection of biological functions. As outlined by previous reports, serum CEACAM1 is dysregulated in distinctive malignant tumours and related with tumour progression. Nonetheless, the serum CEACAM1 expression in non-small-cell lung carcinomas (NSCLC) is unclear. The various expression ratio of CEACAM1-S and CEACAM1-L isoform has seldom been investigated in NSCLC. This study is intended to study the serum CEACAM1 along with the ratio of CEACAM1-S/L isoforms in NSCLC. Methods: The expression of your serum CEACAM1 was determined by enzyme-linked immunosorbent assay. The protein expression as well as the location of CEACAM1 in tumours had been observed by immunohistochemical staining. The CEACAM1 mRNA levels in tumour and standard adjacent tissues had been measured making use of quantitative real-time PCR, plus the expression patterns and the rate of CEACAM1-S and CEACAM1-L have been analysed by reverse transcription-PCR. Outcomes: Serum CEACAM1 levels had been significantly higher in NSCLC sufferers compared with that from normal wholesome controls (P 0.0001). 17 sufferers (81 ) amongst 21 showed higher expression of CEACAM1 by immunohistochemical staining. Despite the fact that no significant variations had been identified involving tumour and standard tissues on mRNA expression levels of CEACAM1 (P 0.05), the CEACAM1-S as well as the CEACAM1-S/L (S: L) ratios have been drastically greater in tumour than regular tissues (P 0.05). Conclusions: Our data indicated that the serum levels of CEACAM1 could discriminate lung cancer individuals from health donors and that CEACAM1 may be a useful marker in early diagnosis of NSCLC. Additionally, our outcomes showed that the expression patterns of CEACAM1 isoforms could possibly be changed during oncogenesis, even when total CEACAM1 in tumour tissues did not show considerable changes.4-Bromo-6-chloropyridin-2-amine Data Sheet Our study suggested that the expression ratios of CEACAM1-S/ CEACAM1-L may possibly be a improved diagnostic indicator in NSCLC than the quantitative changes of CEACAM1.Formula of N-Boc-4-pentyne-1-amine Keyword phrases: Carcinoembryonic antigen-related cell adhesion molecule 1, Non-small-cell lung carcinomas, Enzyme-linked immunosorbent assay, Receiver operating characteristic curve* Correspondence: gao3507@126 Equal contributors 1 Department of Clinical Laboratory, the Sixth People’s Hospital, Shanghai Jiao-tong University School of Medicine, 600 Yi-shan Road, Shanghai 200233, People’s Republic of China two Department of Molecular Biology Laboratory, the Sixth People’s Hospital, Shanghai Jiao-tong University School of Medicine, 600 Yi-shan Road, Shanghai 200233, People’s Republic of China?2013 Zhou et al.PMID:34856019 ; licensee BioMed Central Ltd. This really is an Open Access write-up distributed below the terms of your Inventive Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduc.
